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US 6,428,976 B1Granted
Rapid identification of microorganisms
AssigneeFood Industry Research and Development Institute
InventorsTsung C. Chang, Shiowwen Chen, Hwia C. Ding
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Abstract
A method of determining whether a test microorganism is a known microorganism, involving use of an agent that specifically affects the growth of the known microorganism. The invention also features a method of identifying E. coli O157:H7 that are based on following criteria: a test microorganism is E. coli O157:H7 if the microorganism is (i) E. coli, (ii) incapable of fermenting sorbitol, and (iii) susceptible to infection by AR1 phage.
Claims3
A method of determining whether a test microorganism is E. coli O157:H7, said method comprising:
- providing a first culture that contains the test microorganism and bacteriophage AR1;
- measuring a growth rate-related value of the first culture;
- and
- comparing the value of the first culture with a corresponding growth-rate related value of a second culture, the composition of the second culture being identical to that of the first culture except that the second culture is free of bacteriophage AR1, or contains E. coli O157:H7 and is free of bacteriophage AR1;
- wherein a difference in the two values is an indication that the test microorganism is E. coli O157:H7.
2 more elements
Dependencies are inferred from the claim text.