Preparing artificial organs by forming polylayers of different cell populations on a substrate

An artificial bladder construct comprising:

• a biocompatable substrate;
• a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on the biocompatable substrate;
• and
• a second cultured polylayer of cells derived from a urothelial cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer such that the construct provides the functional equivalent of a natural bladder upon implantation.

A method for producing an artificial organ construct comprising:

• providing a biocompatable substrate in the shape of an organ;
• creating a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on the biocompatable substrate, wherein the first polylayer is attached to the biocompatable substrate;
• and
• creating a second cultured polylayer of cells derived from a second cell population that is different from the smooth muscle cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer such that the construct provides the functional equivalent of a natural biological structure upon implantation, thereby producing an artificial organ construct.

A method for producing an artificial bladder construct comprising:

• providing a biocompatable substrate in the shape of a bladder;
• creating a first cultured polylayer derived from an isolated population of smooth muscle cells on the biocompatable substrate, wherein the first polylayer is attached to the biocompatable substrate;
• and
• creating a second cultured polylayer comprising a urothelial cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer, such that the construct provides the functional equivalent of a natural bladder upon implantation, thereby producing an artificial bladder construct.

A method for treating a subject with a genitourinary disorder comprising:

• providing an artificial organ construct having a first cultured polylayer of cells derived from an isolated population of smooth muscle cells on a biocompatable substrate, and second cultured polylayer of a urothelial cell population, wherein the second polylayer is coupled to the first polylayer at a boundary between the first and second polylayer;
• implanting the organ construct into the subject, such that the construct provides the functional equivalent of a natural structure;
• and
• monitoring the subject for a modulation in the genitourinary disorder.