Oligonucleotide mediated specific cytokine induction and reduction of tumor growth in a mammal
AssigneeHybridon, Inc.
InventorsSudhir Agrawal, Qiuyan Zhao
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Abstract
The invention provides new methods for modulating specific CMI-inducing cytokines in vivo. Such new methods result in stimulation of the cytokines IL-6, IL-12 MIP-1β and MCP without substantially inducing undesired cytokines. The methods according to the invention are based upon administration of oligonucleotides containing particular structural motifs which lead to specific cytokine induction.
Claims1
A method for reducing tumor growth in a mammal which has a tumor, the method comprising administering to a mammal having a tumor in an amount and for a time sufficient to reduce tumor growth an oligonucleotide having the nucleotide sequence Nn1-N2-CpG-N3Nn4, wherein N represents any nucleoside, n1 and n4 each independently represent a number from 0-10 n2 represents a number from 0 to 20 and n3 represents a number from 0-20, such that n2+n3 equals from about 6 to about 40, wherein the underlined region represents a nucleoside phosphodiester or phosphorothioate region or a mixed backbone region having phosphodiester and phosphorothiate nucleosides, wherein CpG represents a cytosine-guanosine dinucleoside phosphorothioate or phosphodiester nucleoside wherein the cytosine has a cytidine base having an unmethylated 5-position, and wherein at least one N region comprises four contiguous guanosine nucleosides.
Dependencies are inferred from the claim text.