Method for silver staining a pathologic sample
OwnerShude Zhong, Taiying Chen +2inventors
InventorsShude Zhong, Taiying Chen, Sunil Kumar Aggarwal, Sheng-Hui Su
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Abstract
A method of silver staining a pathologic sample is disclosed. The method involves the reduction of silver diamine complexes bound to components of the sample by a reducing agent released from a reducing agent carrier. The use of a reducing agent carrier which slowly releases the reducing agent during the reaction permits staining at ambient temperatures.
Claims33
A method for silver staining a pathologic sample, comprising contacting the sample under basic conditions with a silver diamine complex and a reducing agent carrier, whereby the reducing agent released by the carrier reduces silver from the diamine complex, thereby depositing Ag0 in the sample.
A method of silver staining a sample, comprising contacting the sample in the following order with:
- a solution of chromic acid;
- a washing solution;
- a basic solution comprising silver diamine complex and sucrose;
- a washing solution;
- a solution of gold chloride;
- a washing solution;
- a solution of sodium thiosulfate;
- a washing solution;
- and
- a Light Green staining solution.
A silver staining kit comprising:
- an oxidizing agent,
- a silver diamine complex,
- a reducing agent carrier, and
- a reaction termination agent,
- each of which is received within a housing.
Dependencies are inferred from the claim text.