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US 6,423,542 B1Granted
Oligonucleotide mediated nucleic acid recombination
AssigneeMaxygen, Inc.
InventorsAndreas Crameri, Willem P. C. Stemmer, Jeremy Minshull, Steven H. Bass, Mark Welch, Jon E. Ness, Claes Gustafsson, Phillip A. Patten
Life of this patent
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Abstract
Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
Claims7
A method of producing a recombinant nucleic acid, the method comprising:
- (i) transducing a population of cells with a set of chemically synthesized overlapping family gene shuffling oligonucleotides;
- and,
- (ii) permitting recombination to occur between the set of overlapping family gene shuffling oligonucleotides and one or more nucleic acid contained within a plurality of cells of the population of cells, thereby providing a population of recombined nucleic acids within the resulting population of recombinant cells.
Dependencies are inferred from the claim text.