Methods and reagents for quantitation of HLA-DR and CD11b expression on peripheral blood cells
AssigneeBecton, Dickinson and Company
InventorKenneth A. Davis
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Abstract
Improved methods, reagents, and kits for quantitation of HLA-DR and/or CD11b expression on peripheral blood cells are presented. Inclusion of a lysosomotropic amine, such as chloroquine, during staining stabilizes HLA-DR and CD11b expression. Use of a novel anti-CD14 conjugate, anti-CD14-PerCP/CY5.5, permits the ready discrimination of monocytes. The improved methods, reagents, and kits may be used to assess immune competence, and to direct and monitor immunostimulatory therapies in septic patients exhibiting monocyte deactivation.
Claims8
A method of measuring CD11b expression on the surface of human blood cells, comprising:
- contacting a sample containing human blood cells with a lysosomotropic amine and an anti-CD11b antibody;
- and then
- detecting the binding of said anti-CD11b antibody to said cells.
Dependencies are inferred from the claim text.