Alzheimer's disease secretase, APP substrates therefor, and uses thereof
AssigneePharmacia & Upjohn Company
InventorsMark E. Gurney, Michael J. Bienkowski, Robert L. Heinrikson, Luis A. Parodi, Riqiang Yan
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Abstract
The present invention provides the enzyme and enzymatic procedures for cleaving the β secretase cleavage site of the APP protein and associated nucleic acids, peptides, vectors, cells and cell isolates and assays. The invention further provides a modified APP protein and associated nucleic acids, peptides, vectors, cells, and cell isolates, and assays that are particularly useful for identifying candidate therapeutics for treatment or prevention of Alzheimer's disease.
Claims8
A purified polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 73 or a contiguous fragment thereof having apartyl protease activity toward APP,
- wherein said fragment includes amino acids D-T-G at positions 93-95 and amino acids D-S-G at positions 264-966 of SEQ ID NO:
- 73.
A purified polypeptide produced by a process comprising steps of:
- culturing a host cell transformed or transfected with a nucleic acid encoding the polypeptide under conditions wherein the cell expresses the polypeptide encoded by the nucleic acid, wherein the nucleic acid comprises a nucleotide sequence that encodes a polypeptide that comprises the contiguous amino acid sequence set forth in SEQ ID NO:
- 73, and
- isolating the expressed polypeptide.
A purified polypeptide produced by a process comprising steps of:
- culturing a host cell transformed or transfected with a nucleic acid encoding the polypeptide under conditions wherein the cell expresses the polypeptide encoded by the nucleic acid, wherein the nucleic acid comprises a nucleotide sequence encoding a polypeptide that comprises the contiguous amino acid sequence set forth in SEQ ID NO:
- 73 or a contiguous fragment thereof that exhibits aspartyl protease activity toward APP, wherein said fragment includes amino acids D-T-G at positions 93-95 of SEQ ID NO:
- 73 and amino acids D-S-G at positions 264-266 of SEQ ID NO:
- 73, and
- isolating the expressed polypeptide.
Dependencies are inferred from the claim text.