Transgenic plants expressing assembled secretory antibodies
AssigneeThe Scripps Research Institute
InventorsMich B. Hein, Andrew Hiatt
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Abstract
The present invention relates to expression and assembly of foreign multimeric proteins—e.g., antibodies—in plants, as well as to transgenic plants that express such proteins. In one of several preferred embodiments, the generation and assembly of functional secretory antibodies in plants is disclosed. The invention also discloses compositions produced by the transgenic plants of the present invention and methods of using same.
Claims69
A transgenic plant, comprising:
- (a) plant cells containing nucleotide sequences encoding immunoglobulin heavy- and light-chain polypeptides, wherein each polypeptide contains a leader sequence forming a secretion signal;
- and
- (b) immunoglobulin molecules encoded by said nucleotide sequences, wherein said leader sequence is cleaved from said immunoglobulin molecules following proteolytic processing.
A method for making a transgenic plant capable of producing immunoglobulin molecules, comprising:
- (a) introducing into the genome of a first member of a plant species a first mammalian nucleotide sequence encoding an immunoglobulin heavy chain portion-containing polypeptide including a leader sequence forming a secretion signal, to produce a first transformant;
- (b) introducing into the genome of a second member of said plant species a second mammalian nucleotide sequence encoding an immunoglobulin light chain portion-containing polypeptide including a leader sequence forming a secretion signal, to produce a second transformant;
- (c) cross-pollinating said first and second transformants to generate a progeny population;
- and
- (d) isolating from said progeny population a transgenic plant species producing an immunoglobulin molecule, wherein said leader sequence is cleaved from said immunoglobulin molecules following proteolytic processing.
A transgenic plant, comprising:
- (a) plant cells containing nucleotide sequences encoding immunoglobulin heavy- and light-chain polypeptides, wherein each polypeptide contains a leader sequence forming a secretion signal and wherein each nucleotide sequence further comprises the same promoter directing expression of said immunoglobulin heavy- and light-chain polypeptides;
- and
- (b) immunoglobulin molecules encoded by said nucleotide sequences;
- wherein said leader sequence is cleaved following proteolytic processing.
A transgenic plant, comprising:
- (a) plant cells containing nucleotide sequences encoding immunoglobulin heavy- and light-chain polypeptides, wherein each polypeptide contains a leader sequence forming a secretion signal and wherein each nucleotide sequence comprises a functionally identical promoter that controls expression of said immunoglobulin heavy- and light-chain polypeptides;
- and
- (b) immunoglobulin molecules encoded by said nucleotide sequences;
- wherein said leader sequence is cleaved following proteolytic processing.
A transgenic plant, comprising:
- (a) plant cells containing nucleotide sequences encoding immunoglobulin heavy- and light-chain polypeptides, wherein each polypeptide contains a leader sequence forming a secretion signal and wherein each nucleotide sequence comprises a constitutive promoter that controls expression of said immunoglobulin heavy- and light-chain polypeptides;
- and
- (c) immunoglobulin molecules encoded by said nucleotide sequences, wherein said leader sequence is cleaved from said immunoglobulin molecules following proteolytic processing.
Dependencies are inferred from the claim text.