US 6,416,989 B1 · Histidine kinase two-component in Candida albicans

Abstract

The present invention relates to a histidine kinase, two-component gene (CaHK1) from Candida albicans. CaHK1 encodes a 2471 amino acid protein with an estimated molecular mass of 281.8 kDa. Also provided are vectors, host cells, antibodies and recombinant methods for producing the same. The invention further relates agonists and antagonists and to screening methods for identifying agonists and antagonists of CaHK1 polypeptide activity. The invention additionally relates to diagnostic methods for detecting CaHK1 nucleic acids, polypeptides, and antibodies in a biological sample. The present invention further relates to novel antagonists and vaccines for the prevention or attenuation of infection by Candida albicans.

Claims25

Dependent claims

An isolated protein comprising a polypeptide having an amino acid sequence selected from the group consisting of:

(a) amino acid residues 1-971 of SEQ ID NO:2;
(b) amino acid residues 2-971 of SEQ ID NO:2;
(c) amino acid residues 482-721 of SEQ ID NO:2;
(d) amino acid residues 834-971 of SEQ ID NO:2;
(e) amino acid residues 482-971 of SEQ ID NO:2;
(f) amino acid residues 1-2471 of SEQ ID NO:4;
(g) amino acid residues 2-2471 of SEQ ID NO:4;
(h) amino acid residues 1982-2221 of SEQ ID NO:4;
(i) amino acid residues 2334-2471 of SEQ ID NO:4;
and
(j) amino acid residues 1982-2471 of SEQ ID NO:4.

8 more elements

Dependencies are inferred from the claim text.

Facts

Application

09/419,291Filed 15 Oct 1999 · division

Priority

10 Jul 1997

Granted

9 Jul 2002

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 9/1223
Transferring phosphorus containing groups, e.g. kinases: phosphotransferases with a nitrogenous group as acceptor
C12Y 207/13001
Protein-histidine kinases: protein-histidine pros-kinase
A61K 39/00
Medicinal preparations containing antigens or antibodies
C12N
Microorganisms or enzymes

Family

Application60/052,273Us provisional application · filed 10 Jul 1997

Application60/074,308Us provisional application · filed 11 Feb 1998

Application09/112,450Parent of this division · filed 9 Jul 1998

US 6,120,999 AParent of this division · filed 19 Sep 2000

US 6,416,989 B1This patent

From the US filing history. Foreign family members aren’t listed.

Histidine kinase two-component in Candida albicans

Life of this patent

Histidine kinase two-component in Candida albicans

Life of this patent

Abstract

Claims25