Method of using multicellular particulates to analyze malignant or hyperproliferative tissue
AssigneePrecision Therapeutics, Inc.
InventorPaul L. Kornblith
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Abstract
A comprehensive and integrated system for monitoring (identifying, tracking and analyzing) an individual patient's malignancy or hyperproliferative syndrome through the duration of a malignancy or hyperproliferative syndrome as to a specific patient is provided. Specimens of a patient's cells are collected and mechanically separated into cohesive multicellular particulates. A tissue culture monolayer is grown from the multicellular particulates to form a prime culture, and the tissue culture is monitored over a period of time. The invention allows for study of the effect of various treatment methods on cellular production of vascular endothelial growth factor.
Claims22
A method of observing the behavior of cells derived from a sample of cancer cells, comprising:
- (a) collecting a specimen of a patient's malignant or hyperproliferative cells;
- (b) mechanically separating said specimen into cohesive multicellular particulates having a particle size between 0.25-1.5 mm3;
- (c) growing a tissue culture monolayer from said multicellular particulates to form a prime culture which may be maintained;
- (d) forming at least one subculture of said prime culture for further analysis;
- and
- (e) monitoring said prime culture over a period of time for its characteristics to observe the behavior of the cells in said prime culture.
Dependencies are inferred from the claim text.