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US 6,413,769 B1Granted
α(1,3) galactosyltransferase negative porcine cells
AssigneesUniversity of London, The General Hospital Corporation
InventorsKenth T. Gustafsson, David H. Sachs, Manfred W. Baetscher
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Abstract
Transgenic swine in which the normal expression of α(1, 3) galactosyltransferase is prevented in at least one organ of tissue type. The absence or inactivation of this enzyme prevents the production of carbohydrate moieties having the distinctive terminal Galα1-3Galβ1-4GlcNAc epitope that is a significant factor in xenogeneic, particularly human, transplant rejection of swine grafts.
Claims4
A product, comprising:
- isolated porcine cells in which the normal expression of œ (1,3) galactosyltransferase is inhibited, said cells including a galactosyltransferase gene encoding a polypeptide comprising the amino acid sequence of SEQ. ID NO:
- 1, wherein said gene has been disrupted to inhibit normal expression of œ(1,3) galactosyltransferase.
A process for producing isolated porcine cells that normally do not express galactosyltransferase, comprising:
- disrupting in isolated porcine cells a galactosyltransferase gene encoding a polypeptide comprising the sequence of SEQ ID NO:
- 1 by homologous recombination with DNA isogenic to said gene, said isogenic DNA including a mutation that disrupts said gene to prevent normal expression of galactosyltransferase.
Dependencies are inferred from the claim text.