Protein capable of catalyzing transamination stereoselectively, gene encoding said protein and use thereof

An isolated protein comprising an amino acid sequence represented by SEQ ID NO. 1, wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine, and wherein said protein has a single amino acid substitution within Sequence ID No. 1.

An isolated protein comprising an amino acid sequence which has at least 60% sequence identity to SEQ ID NO. 1, wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine and wherein said protein has a molecular weight of about 37 kDa as a monomer, and is obtainable from Mycobacterium aurum SC-S432.

An isolated protein comprising an amino acid sequence which has at least 90% sequence identity to SEQ ID NO. 1, wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine.

An isolated protein comprising an amino acid sequence which has at least 80% sequence identity to SEQ ID NO. 1, wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine, and wherein said protein has a molecular weight of about 37 kDa as a monomer.

An isolated protein comprising an amino acid sequence which has at least 80% sequence identity to SEQ ID NO. 1, wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine, and wherein said protein is derived from a microorganism belonging to the genus Mycobacterium.

An isolated protein comprising an amino acid sequence represented by amino acids 23 to 339 of SEQ ID NO. 1, wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine.