Methods and host cells for improved cell culture
AssigneeImmunex Corporation
InventorsArvia E. Morris, Pranhitha Reddy
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Abstract
The invention provides improved methods of recombinant protein production in cell culture. More specifically, the invention relates to the modulation of the IGF-1 signaling pathway in cells so as to improve production characteristics.
Claims40
An eukaryotic host cell genetically engineered to express:
- a gene for a protein of interest, wherein the protein of interest is expressed as an extracellular product;
- and
- at least one IGF-1 signaling pathway gene selected from the group consisting of a PKB gene and a MEK gene.
A method of producing a protein of interest, the method comprising culturing an eukaryotic host cell genetically engineered to express:
- a gene for a protein of interest, wherein the protein of interest is expressed as an extracellular product;
- and
- at least one IGF-1-signaling pathway gene selected from the group consisting of a PKB gene and a MEK gene,
- under conditions such that the protein of interest is expressed.
A method of producing an eukaryotic cell for production of a protein of interest, the method comprising genetically engineering an eukaryotic cell
- to express a gene that encodes the protein of interest, wherein the protein of interest is expressed as an extracellular product, and
- to express at least one IGF-1-signaling pathway gene selected from the group consisting of a PKB gene and a MBK gene.
A method of producing a mammalian cell line capable of growth in serum-free medium, the method comprising exposing cells that have been genetically engineered to overexpress at least one IGF-1 signaling pathway gene selected from the group consisting of a PKB gene and a MEK gene to serum-free medium, and isolating a cell line that grows in serum-free medium.
A method of producing an eukaryotic cell for production of a protein of interest, the method comprising genetically engineering an eukaryotic cell to express the protein of interest, wherein the protein of interest is expressed as an extracellular product, and wherein the eukaryotic cell has been genetically engineered to express an IGF-1 signaling pathway gene selected from the group consisting of a PKB gene and a MEK gene.
A method of producing an eukaryotic cell for production of a protein of interest, the method comprising genetically engineering an eukaryotic cell to express an IGF-1 signaling pathway gene selected from the group consisting of a PKB gene and a MEK gene, wherein the eukaryotic cell expresses the protein of interest, wherein the protein of interest is expressed as an extracellular product.
Dependencies are inferred from the claim text.