Recombinant gelatin and full-length triple helical collagen
AssigneesCohesion Technologies, Inc., Genotypes, Inc.
InventorsDavid R. Olsen, Robert Chang, Hugh McMullin, Ronald A. Hitzeman, George Chisholm
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Abstract
Methods are disclosed for simplified recombinant production of fibrillar collagens. DNAs encoding fibrillar collagen monomers lacking the N propeptide, the C propeptide, or both propeptides are introduced into recombinant host cells and expressed. Trimeric collagen is recovered from the recombinant host cells.
Claims29
A method for the production of fibrillar collagen comprising:
- culturing a recombinant host cell comprising a DNA encoding a fibrillar collagen monomer lacking a C propeptide SSAD under conditions appropriate for expression of said DNA;
- and
- producing fibrillar collagen, wherein said DNA encodes a fibrillar collagen selected from the group consisting of collagen α1(I) and collagen α2(I).
A method for the production of fibrillar procollagen, comprising:
- culturing a recombinant yeast host cell comprising a DNA encoding a fibrillar collagen monomer lacking a N propeptide under conditions appropriate for expression of said DNA;
- and
- producing fibrillar collagen, wherein said DNA encodes a fibrillar collagen selected from the group consisting of collagen α1(I) and collagen α2(I).
A recombinant host cell comprising:
- an expression construct comprising DNA encoding a fibrillar collagen monomer lacking a C propeptide SSAD, wherein said DNA encodes a fibrillar collagen selected from the group consisting of collagen α1(I) and collagen α2(I).
A method of producing telopeptide collagen fibrils, comprising:
- culturing a recombinant host cell comprising a DNA encoding a fibrillar collagen monomer lacking a C propeptide and a N propeptide under conditions appropriate for expression of said DNA, thereby producing fibrillar collagen;
- recovering said fibrillar collagen;
- and
- forming fibrils from said fibrillar collagen, wherein said DNA encodes a fibrillar collagen selected from the group consisting of collagen α1(I) and collagen α2(I).
A method of producing gelatin, comprising:
- culturing a recombinant host cell comprising a DNA encoding a collagen monomer lacking a N or C propeptide under conditions appropriate for expression of said DNA;
- and
- producing gelatin, wherein said DNA encodes a fibrillar collagen selected from the group consisting of collagen α1(I) and collagen α2(I).
Dependencies are inferred from the claim text.