US 6,411,904 B1 · Zero dead time architecture for flow cytometer

Abstract

A method and apparatus for providing a zero dead time for flow analyzers, flow cytometers, and other measurement devices. A circular buffer is used to store data from a flow analyzer in a plurality of data storage areas, until it is convenient to implement more time consuming data processing procedures. User specified parameters, including sampling rate and/or sampling period, size and number of data storage areas, size of the circular or other buffer, signal-to-noise threshold, order of processing when a plurality of Digital Signal Processors (DSPs) is used, and fixed trailing distance, are used to provide flexible and convenient operation by a user. The probability of missing a rare event occurring within the laser beam or other light beam of a flow analyzer or other measurement device is reduced to zero. Functional equivalents may be used in place of the circular buffer, for example, First In-First Out buffers which route data to a cache for potential re-reading and/or reprocessing or additional processing of data, cascading buffers, etc. Circular and functionally equivalent buffers permit sophisticated data processing in which preceding data (for example, data not meeting the S/N threshold) subsequently are re-analyzed for the possibility that the data are part of a developing event peak, such as a developing shoulder thereof, or part of a declining peak. In addition, logic and algorithms to permit re-examination and potential reprocessing of “old” subthreshold data is utilized to improve the S/N ratio of an event peak.

Claims23

Dependent claims

A data collection and measurement device collecting data with substantially zero dead time, comprising:

a) a circular buffer comprising:
a plurality of data storage areas that are linked to receive and consecutively store incoming data from a plurality of successive sampling periods for at least one event of a measurement device, where each data storage area is configured to receive and store data from one sampling period, and the data storage areas are linked in an order that provides storage for data from a next-in-time sampling period into the next-in-order data storage area;
a First pointer;
and
a Second pointer;
and
b) at least one Digital Signal Processor (DSP), where the First Pointer directs receipt and storage of data of the next-in-time sampling period into the next-in-order data storage area, and, once all data storage areas contain data, directs receipt of data of the next-in-time sampling period, and storage by overwriting therewith data in the next-in-order data storage area, the Second Pointer is directed to the data storage area that stores data from a sample period preceding a current time of data receipt and storage by a fixed trailing distance as a referenced to the First Pointer, and directs one of the at least one DSPs to read and process data in the data storage area to which the Second Pointer is directed, and wherein once the next-in-time data have been stored, the First Pointer advances to the next-in-order data storage area, and once data in the data storage area to which the Second Pointer is directed have been processed, the Second Pointer advances to the next-in-order data storage area,
wherein said collection and measurement apparatus collects and processes data from the at least one event, and where the fixed trailing distance equals at least a longest possible time for an event.

5 more elements

A flow analyser collecting data with substantially zero dead time for at least one event, comprising:

an energy beam in communication with a particle to be measured, said particle in an examination zone for a predetermined period of time;
a sensor configured to measure a particle signal emitted as a result of said energy beam being communicated to said particle;
a circular buffer coupled to said sensor configured to read and store a digital signal representing data associated with said particle signal, wherein said circular buffer comprises,
a plurality of data storage areas that are linked to receive and consecutively store incoming data from a plurality of successive sampling periods for at least one event of said sensor, where each data storage area is configured to receive and store data from one sampling period, and the data storage areas are linked in an order that provides storage for data from a next-in-time sampling period into the next-in-order data storage area data storage area;
and a first pointer;
and a second pointer;
and at least one Digital Signal Processor (DSP), where the First Pointer directs receipt and storage of data of the next-in-time sampling period into the next-in-order data storage area, and, once all data storage areas contain data, directs receipt of data of the next-in-time sampling period, and storage by overwriting therewith data in the next-in-order data storage area, the Second Pointer is directed to the data storage area that stores data from a sample period preceding a current time of data receipt and storage by a fixed trailing distance as a referenced to the First Pointer, and directs one of the at least one DSPs to read and process data in the data storage area to which the Second Pointer is directed, and wherein once the next-in-time data have been stored, the First Pointer advances to the next-in-order data storage area, and once data in the data storage area to which the Second Pointer is directed have been processed, the Second Pointer advances to the next-in-order data storage area, wherein said collection and measurement apparatus collects and processes data from the at least one event, and where the fixed trailing distance equals at least a longest possible time for an event.

4 more elements

Dependencies are inferred from the claim text.

Zero dead time architecture for flow cytometer

Life of this patent

Zero dead time architecture for flow cytometer

Life of this patent

Abstract

Claims23