Laundry detergent and/or fabric care compositions comprising a modified transferase
AssigneeThe Procter & Gamble Company
InventorsJohan Smets, Mary Vijayarani Barnabas, Michael Stanford Showell, Stanton Lane Boyer, André Christian Convents
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Abstract
The present invention relates to a modified enzyme which comprises a catalytically active amino acid sequence of a transferase linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD). The present invention further relates to laundry detergent and/or fabric care compositions comprising such modified enzyme, for improved fabric care and cleaning benefits.
Claims38
A modified enzyme which comprises a catalytically active amino acid sequence of a transferase linked to an amino acid sequence comprising a cellulose binding domain via a linking region selected from the group consisting of, Humicola insolens family 45 cellulase linker, NifA gene of Klebsiella pneumonia-CiP linker, E. coli OmpA gene-CiP linker, E3 cellulase Thermomonospora fusca linker, CenA cellulase linker, polyethylene glycol derivatives, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-ethyl-5-phenylisoaxolium-3-sulphonate, N-ethoxycarbonyl-2-ethoxy 1,2,dihydroquinoline or glutaraldehyde, crosslinkers and combinations thereof;
- wherein said transferase is selected from the group consisting of:
- EC 2.1.1.15, EC 2.1.1.18, EC 2.1.2.1, EC 2.1.2.4, EC 2.2.1.3, EC 2.3.1.3, EC 2.3.1.18, EC 2.3.1.57, EC 2.3.1.75, EC 2.3.1.79, EC 2.3.1.84, EC 2.3.1.88, EC 2.3.1.96, EC 2.3.1.142, EC 2.4.1.2, EC 2.4.1.4, EC 2.4.1.5, EC 2.4.1.9, EC 2.4.1 .10, EC 2.4.1.11, EC 2.4.1.12, EC 2.4.1.13, EC 2.4.1.16, EC 2.4.1.18, EC 2.4.1.21, EC 2.4.1 24, EC 2.4.1.25, EC 2.4.1.29, EC 2.4.1.34, EC 2.4.1.35, EC 2.4.1.49, EC 2.4.1.67, EC 2.4.1.71, EC 2.4.1.75, EC 2.4.1.82, EC 2.4.1.90, EC 2.4.1.93, EC 2.4.1.99, EC 2.4.1.100, EC 2.4.1.113, EC 2.4.1.121, EC 2.4.1.125, EC 2.4.1.140, EC 2.4.1.161, EC 2.4.1.168, EC 2.4.1.169, EC 2.4.1.183, EC 2.5.1.10, EC 2.5.1.20, EC 2.6.1, 2.3.2.13, xyloglucan endotransglycosylase (XET), endo-xyloglucan transferase (EXGT) and combinations thereof.
A modified enzyme which comprises a catalytically active amino acid sequence of a transferase linked to an amino acid sequence comprising a cellulose binding domain via a linking region selected from the group consisting of:
- Humicola insolens family 45 cellulase linker, NifA gene of Klebsiella pneumoniae-CiP linker, E. coli OmpA gene-CiP linker, E3 cellulase Thermomonospora fusca linker, CenA cellulase linker, polyethylene glycol derivatives, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-ethyl-5-phenylisoaxolium-3-sulphonate, N-ethoxycarbonyl-2-ethoxy, 1,2 dihydroquinoline or glutaraldehyde, crosslinkers and combinations thereof;
- wherein said transferase is a mutant glycosyl transferase selected from the group consisting of:
- EC 2.4.1.2, EC 2.4.1.4, EC 2.4.1.5, EC 2.4.1.9, EC 2.4.1.10, EC 2.4. 1.11, EC 2.4.1.12, EC 2.4.1.13, EC 2.4.1.16, EC 2.4.1.18, EC 2.4.1.21, EC 2.4.1.24, EC 2.4.1.25, EC 2.4.1.29, EC 2.4.1.34, EC 2.4.1.35, EC 2.4.1.49, EC 2.4.1.67, EC 2.4.1.71, EC 2.4.1.75, EC 2.4.1.82, EC 2.4.1.90, EC 2.4.1.93, EC 2.4.1.99, EC 2.4.1.100, EC 2.4.1.113, EC 2.4.1.121, EC 2.4.1.125, EC 2.4.1.140, EC 2.4.1.161, EC 2.4.1.168, EC 2.4.1.168, EC 2.4.1.169, EC 2.4.1.183 and mixture thereof.
Dependencies are inferred from the claim text.