Method and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules
AssigneeUniversity of Michigan
InventorH. David Humes
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Abstract
Methods, including culture media conditions, which provide for isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis are disclosed. The methods rely on culturing adult kidney cells in a culture media treated with combinations of transforming growth factor-β1, epidermal growth factor, and all-trans retinoic acid.
Claims3
An isolated and purified population of renal stem cells which has been cultured and expanded ex vivo, wherein the cells have been passaged at least three times, wherein the population retains renal stem cells after at least three passages, and wherein the stem cells are capable of differentiating into renal proximal tubule cells.
Dependencies are inferred from the claim text.