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US 6,410,309 B1Granted
Cell culture apparatus and methods of use
AssigneeBioCrystal, Ltd.
InventorsEmilio Barbera-Guillem, M. Bud Nelson
Life of this patent
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Abstract
Provided is a cell culture apparatus for culturing cells, and optionally, for performing magnetic separation of cells desired to be cultured. The cell culture apparatus preferably comprises a frame; at least one gas permeable membrane securedly sealed in a leak-proof sealing to a frame and an opposing surface comprising a rigid surface in forming a culture chamber therebetween; and at least one resealable aperture to allow substances to be introduced into, or withdrawn from, the culture chamber.
Claims22
A cell culture apparatus comprising:
- a frame comprising a housing, and at least one aperture which comprises an opening through the frame, wherein each aperture of the at least one aperture contains a septum;
- at least one gas permeable membrane, wherein the at least one gas permeable membrane is securedly sealed in a leak-proof sealing to the frame in forming a portion of a wall of a culture chamber;
- a rigid surface which forms a wall of the culture chamber, wherein the culture chamber is formed between the at least one gas permeable membrane and the rigid surface and the frame, and wherein the at least one gas permeable membrane is optically transparent and clear.
Dependencies are inferred from the claim text.