Serum-free medium for culturing animal cells
AssigneesChugai Seiyaku Kabushiki Kaisha, Irvine Scientific Sales Company, Inc.
InventorsKazushi Shibuya, Masaru Atsumi, Shigeyuki Tsunakawa, Kaneo Nogaki, Thomas Reid Fletcher, Katsuyuki Imada, Bjorn Kenneth Lydersen
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Abstract
The present invention relates to a serum-free medium for culturing animal cells which contains soybean protein hydrolysate and yeast extract; a method for culturing animal cells which comprises a step of culturing animal cells in the serum-free medium; and a method for producing a desired substance which comprises a step of culturing animal cells in the serum-free medium, causing the desired substance to be produced by and secreted out of the animal cells and a step of isolating the desired substance from the serum-free medium.
Claims20
A serum-free medium for culturing animal cells (A) that contains soybean protein hydrolysate, yeast extract and wheat protein hydrolysate and (B) that promotes production of a recombinant protein or peptide.
A method for culturing animals cells which comprises culturing animal cells in a serum-free medium (A) that contains soybean protein hydrolysate, yeast extract and wheat protein hydrolysate, and (B) that promotes production of a recombinant protein or peptide.
A method for producing a substance which comprises
- (1) culturing animal cells in a serum-free medium (A) that contains soybean protein hydrolysate, yeast extract and wheat protein hydrolysate, and (B) that promotes production of a recombinant protein or peptide, causing said substance to be produced by and secreted out of said animals cells, and
- (2) isolating said substance from said serum-free medium.
A serum-free medium for culturing animal cells that contains soybean protein hydrolysate, wheat protein hydrolysate and yeast extract, wherein the ratio by weight of the amount of added soybean protein hydrolysate to the ratio by weight of the amount of added yeast extract is in the range of 50:50 to 90:10.
Dependencies are inferred from the claim text.