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US 6,403,312 B1Granted
Protein design automatic for protein libraries
AssigneeXencor, Inc.
InventorsBassil I. Dahiyat, Robert J. Hayes, Jörg Bentzien, Klaus M. Fiebig
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Abstract
The invention relates to the use of protein design automaton (PDA) to generate computationally prescreened secondary libraries of proteins, and to methods and compositions utilizing the libraries.
Claims8
A method for generating a secondary library of scaffold protein variants comprising:
- a) providing a primary library comprising a computationally rank-ordered list of scaffold protein primary variant sequences;
- b) generating a list of primary variant positions in said primary library;
- c) combining a plurality of said primary variant positions to generate a secondary library of secondary sequences.
A method for generating a secondary library of scaffold protein variants comprising:
- a) providing a primary library comprising a computationally rank-ordered list of scaffold protein primary variant sequences comprising primary variant positions;
- and,
- b) combining a plurality of said primary variant positions to generate a secondary library of secondary sequences.
A method for generating a secondary library of scaffold protein variants comprising:
- a) providing a primary library comprising a computationally rank-ordered list of scaffold protein primary variant sequences;
- b) generating a probability distribution table of amino acid residues in a plurality of variant positions;
- c) combining a plurality of said amino acid residues to generate a secondary library of secondary sequences.
A method for generating a secondary library of scaffold protein variants comprising:
- a) providing a first library computationally rank-ordered list of scaffold protein primary variants;
- b) generating a probability distribution of amino acid residues in a plurality of variant positions;
- c) synthesizing a plurality of scaffold protein secondary variants comprising a plurality of said amino acid residues to forma secondary library;
- wherein at least one of said secondary variants is different from said primary variants.
1 more element
Dependencies are inferred from the claim text.