Testing multiple fluid samples with multiple biopolymer arrays
AssigneeAgilent Technologies, Inc.
InventorsSueAnn C. Dahm, Arthur Schleifer, Carol T. Schembri, Douglas A. Amorese
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Abstract
A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.
Claims26
A method of testing multiple fluid samples with multiple biopolymer arrays to detect a binding pattern between the multiple fluid samples and the arrays, comprising:
- (a) assembling a cover to a one-piece substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover an the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port which includes a resilient self-sealing portion;
- (b) introducing the multiple fluid samples into respective chambers through a port of each such that the fluid samples contact respective arrays;
- and
- (c) observing a binding pattern on the arrays;
- wherein each chamber is accessible through a first and a second port each of which includes a resilient self-sealing portion, the method additionally comprising inserting a first set of conduits through the resilient members of respective first ports, and inserting a second set of conduits through the self-sealing portion of respective second ports, and wherein the multiple fluid samples are introduced into each chamber through the first set of conduits while venting occurs through the second set of conduits.
A method of testing multiple fluid samples with multiple biopolymer arrays to detect a binding pattern between the multiple fluid samples and the arrays, comprising:
- (a) assembling a cover to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port;
- (b) introducing the multiple fluid samples into respective chambers through a port of each such that the fluid samples contact respective arrays;
- and
- (c) observing a binding pattern of the arrays;
- wherein each chamber is accessible through a first and a second port, and wherein fluid samples are introduced into respective chambers through respective first ports while venting through respective second ports.
A method of testing multiple fluid samples with multiple biopolymer arrays to detect a binding pattern between the multiple fluid samples and the arrays, using a cover which includes a cover member and a resilient gasket with multiple openings, the method comprising:
- (a) assembling the cover to a contiguous substrate carrying on a first side multiple arrays each with multiple regions of biopolymers linked to the substrate, with the gasket sandwiched between the substrate and cover member and the gasket openings aligned with respective arrays, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and being accessible through a port comprising respective port portions of the resilient gasket which normally close the port;
- (b) penetrating gasket port portions with at least one conduit and introducing fluid samples into respective chambers through the at least one conduit and chamber ports such that the fluid samples contact respective arrays;
- and
- (c) observing a binding pattern of the arrays.
An apparatus for testing multiple fluid samples with multiple biopolymer arrays, comprising:
- (a) a cover defining multiple cavities on a first side and with respective ports communicating with the cavities, the ports including respective resilient self-sealing portions normally closing the ports, which cover can be assembled to a one-piece planar substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array;
- wherein the cover has a first and a second set of ports such that each chamber is accessible through a first and a second port so that fluid samples can be introduced into respective chambers through respective first ports while venting through respective second ports.
An apparatus for testing multiple fluid samples with multiple biopolymer arrays, comprising:
- a cover including:
- a resilient gasket with multiple openings and port portions;
- and a cover member
- wherein the gasket and cover member are dimensioned so that the cover can be assembled to a contiguous planar substrate carrying on a first side multiple arrays each with multiple regions of biopolymers linked to the substrate, with the gasket sandwiched between the substrate and cover member and with the gasket openings aligned with respective arrays, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each accessible through a port which includes the gasket port portions normally closing the port, upon penetration of a conduit through respective port portions of the resilient gasket.
Dependencies are inferred from the claim text.