Human papilloma virus vectors
AssigneeMaxygen, Inc.
InventorsDoris Apt, Paul Khavari, William P. C Stemmer
Life of this patent
Estimating the term…
Abstract
The invention provides human papillomavirus vectors useful in gene therapy. Such a vector contains E1 and E2 coding regions from a benign or low-risk human papillomavirus operably linked to a promoter and enhancer, and an LCR region from a human papillomavirus comprising an origin of replication including binding sites for the E1 and E2 proteins. The invention further provides methods of using such vectors in gene therapy, methods of controlling expression using a patch, and methods of using such vectors to evolve drugs for stimulation of hair growth or alteration of hair color.
Claims40
A vector comprising:
- a) E1 and E2 coding regions of a benign or low-risk human papilloma virus (HPV) operably linked to a promoter and enhancer;
- and
- b) a long control region (LCR) of an HPV comprising:
- i) an origin of replication;
- and
- ii) binding sites for E1 and E2 proteins encoded by said E1 and E2 coding regions;
- wherein the LCR and E1 and E2 coding regions are present in a contiguous segment.
A vector comprising:
- a) E1 and E2 coding regions of a benign or low-risk human papilloma virus (HPV) operably linked to a first promoter and enhancer;
- b) a long control region (LCR) of an HPV comprising:
- i) an origin of replication;
- and
- ii) binding sites for E1 and E2 proteins encoded by said E1 and E2 coding regions;
- wherein the LCR and E1 and E2 coding regions are present in a contiguous segment;
- and
- c) a nucleic acid sequence encoding an exogenous protein operably linked to a second promoter and enhancer.
A vector comprising:
- a) E1 and E2 coding regions of a benign or low-risk human papilloma virus (HPV) operably linked to their natural promoter and enhancer;
- and
- b) a long control region (LCR) of an HPV comprising:
- i) an origin of replication;
- and
- ii) binding sites for E1 and E2 proteins encoded by said E1 and E2 coding regions.
A vector comprising:
- a) an early open reading frame (ORF) of a benign or low-risk human papilloma virus (HPV) comprising an E1 ORF and E2 ORF each operably linked to a promoter, and
- b) a long control region (LCR) of an HPV comprising:
- i) an origin of replication;
- and
- ii) binding sites for E1 and E2 proteins encoded by said E1 ORF and E2 ORF,
- wherein the LCR and E1 ORF and E2 ORF are present in a contiguous segment.
A vector comprising:
- a) E1 and E2 coding regions of a benign or low-risk human papilloma virus (HPV) operably linked to a promoter and enhancer;
- b) a long control region (LCR) of an HPV comprising:
- i) an origin of replication;
- and
- ii) binding sites for E1 and E2 proteins encoded by said E1 and E2 coding regions, wherein the origin of replication and the promoter are present in a contiguous segment.
A vector comprising:
- a) E1 and E2 coding regions of a benign or low-risk human papilloma virus (HPV) operably linked to a promoter, and
- b) a long control regions (LCR) of an HPV comprising:
- i) an origin of replication;
- and
- ii) binding sites for E1 and E2 pins encoded by said E1 and E2 coding regions;
- wherein the E1 and E2 coding regions are produced by shuffling an early region of HPV-2, HPV-27, and HPV-57.
A vector comprising:
- a) E1 and E2 coding regions of a benign or low risk human papilloma virus (HPV) operably linked to a first promoter;
- b) a long control region (LCR) of an HPV comprising:
- i) an origin of replication;
- and
- ii) binding sites for E1 and E2 proteins encoded by said E1 and E2 coding regions;
- wherein the E1 and E2 coding regions are produced by shuffling an early region of HPV-2, HPV-27, and HPV-57;
- and
- c) a nucleic acid sequence encoding an exogenous protein operably linked to a second promoter.
A vector comprising:
- a) an E1 coding region of a benign or low-risk human papilloma virus (HPV) operably linked to a first promoter and enhancer;
- b) an E2 coding region of a benign or low-risk human papilloma virus (HPV) operably linked to a second promoter and enhancer;
- and
- c) a long control region (LCR) of an HPV comprising:
- i) an origin of replication;
- and
- ii) binding sites for E1 and E2 proteins encoded by said E1 and E2 coding regions;
- wherein the LCR and the first or second promoter are present in a contiguous segment.
The vector of 26, further comprising a nucleic acid sequence encoding an exogenous protein operably linked to a third promoter and enhancer.
A method of expressing an exogenous protein in human skin in vitro comprising:
- introducing a vector into human skin in vitro wherein said vector comprises:
- a) E1 and E2 coding regions of a benign or low-risk human papilloma virus (HPV) operably linked to a first promoter and enhancer;
- b) a long control region (LCR) of an HPV comprising:
- i) an origin or replication;
- and
- ii) binding sites for the E1 and E2 proteins encoded by said E1 and E2 coding regions;
- and
- c) a nucleic acid sequence encoding an exogenous protein operably linked to a second promoter and enhancer,
- such that the exogenous protein is expressed to a detectable level in at least one cell of said human skin.
Dependencies are inferred from the claim text.