Rupestris stem pitting associated virus nucleic acids, proteins, and their uses
AssigneeProfiGen Inc.
InventorsDennis Gonsalves, Baozhong Meng
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Abstract
The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide of a Rupestris stem pitting associated virus. The encoding DNA molecule, either alone in isolated form, in an expression system, a host cell, or a transgenic grape plant, is also disclosed. Other aspects of the present invention relate to a method of imparting Rupestris stem pitting associated virus resistance to grape plants by transforming them with the DNA molecule of the present invention, and a method of detecting the presence of a Rupestris stem pitting associated virus, such as RSPaV-1, in a sample.
Claims8
Plant material identified according to a method comprising the steps of:
- (a) providing a sample from a plant suspected of being infected with Rupestris stem pitting associated virus,
- (b) contacting the sample with a nucleic acid probe comprising a Rupestris stem pitting virus nucleic acid molecule under conditions that allow for the hybridization of the probe to a Rupestris stem pitting associated virus nucleic acid molecule, wherein an undetectable hybridization signal indicates that the plant is not infected with a Rupestris stem pitting associated virus, and
- (c) selecting a plant that is not infected with a Rupestris stem pitting associated virus.
Dependencies are inferred from the claim text.