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US 6,395,958 B1Granted
Method of producing a polypeptide in an ungulate
AssigneeInfigen, Inc.
InventorsNikolai S. Strelchenko, Jeffrey M. Betthauser, Gail L. Jurgella, Marvin M. Pace, Michael D. Bishop
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Abstract
The present invention relates to cloning technologies. The invention relates in part to immortalized and totipotent cells useful for cloning animals, the embryos produced from these cells using nuclear transfer techniques, animals that arise from these cells and embryos, and materials, methods, and processes for establishing such cells, embryos, and animals.
Claims16
A method of producing a polypeptide in au ungulate, the method comprising:
- (a) forming a nuclear transfer embryo by translocating a cultured ungulate nonembryonic cell, or a nucleus thereof, into an enucleated oocyte obtained from the same species as the ungulate nonembryonic cell, wherein said ungulate nonembryonic cell is, or is obtained by culture of, a cell transfected in vitro with a DNA sequence encoding said polypeptide operably linked to a milk protein promoter, such that said DNA sequence integrates into the genome of said cell;
- (b) before, during, or after said forming step, activating said oocyte, whereby an activated nuclear transfer embryo is provided following said forming and activating steps;
- and
- (c) transferring said activated nuclear transfer embryo, or a recloned embryo thereof, into the uterus of a host ungulate of the same species as the ungulate nonembryonic cell so as to produce a fetus that undergoes fill fetal development and parturition to generate said ungulate, wherein said ungulate expresses said DNA sequence and produces said polypeptide in milk, blood, serum, or plasma in detectable quantities.
1 more element
Dependencies are inferred from the claim text.