Phosphoserine phosphatase gene of coryneform bacteria
AssigneeAjinomoto Co., Inc.
InventorsMikiko Suga, Yoko Asakura, Masakazu Sugimoto, Hisao Ito
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Abstract
The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.
Claims15
An isolated DNA coding for a protein which comprises an amino acid sequence of SEQ ID NO:
- 2.
An isolated DNA coding for a protein having phosphoserine phosphatase activity, which hybridizes to a DNA sequence encoding SEQ ID NO:
- 2 under the stringent conditions comprise washing at 60° C. and at a salt concentration corresponding to 1×SSC and 0.1% SDS, wherein said protein has homology of not less than 80% to the amino acid sequence of SEQ ID NO:
- 2.
An isolated DNA which is DNA as defined in the following (a) or (b):
- (a) A DNA which comprises a nucleotide sequence corresponding to nucleotide numbers of 210-1547 of nucelotide sequence of SEQ ID NO:
- 1;
- or
- (b) A DNA which is hybridizable with a probe which comprises the nucleotide sequence corresponding to nucleotide numbers of 210-1547 of nucleotide sequence of SEQ ID NO:
- 1 under stringent conditions which comprise washing at 60° C. and at a salt concentration corresponding to 1×SSC and 0.1% SDS, which encodes a protein having homology of not less than 80% to the amino acid sequence of SEQ ID NO:
- 2, and which codes for the protein having the phosphoserine phosphatase activity.
Dependencies are inferred from the claim text.