Detection of Rupestris stem pitting associated virus
AssigneeCornell Research Foundation, Inc.
InventorsDennis Gonsalves, Baozhong Meng
Life of this patent
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Abstract
The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide of a Rupestris stem pitting associated virus. The encoding DNA molecule, either alone in isolated form, in an expression system, a host cell, or a transgenic grape plant, is also disclosed. Other aspects of the present invention relate to a method of imparting Rupestris stem pitting associated virus resistance to grape plants by transforming them with the DNA molecule of the present invention, and a method of detecting the presence of a Rupestris stem pitting associated virus, such as RSPaV-1, in a sample.
Claims40
A method for determining whether a sample includes a Rupestris stem pitting associated virus, said method comprising:
- contacting the sample with a Rupestris stem pitting associated virus-specific nucleic acid probe under conditions that allow for hybridization of the probe to a Rupestris stem pitting associated virus nucleic acid molecule, wherein a detectable hybridization signal indicates that Rupestris stem pitting associated virus is present in the sample.
A method for determining whether a Rupestris stem pitting associated virus is present in a sample, said method comprising:
- contacting the sample with a Rupestris stem pitting associated virus-specific nucleic acid probe under conditions that allow for hybridization of the probe to a Rupestris stem pitting associated virus nucleic acid molecule, wherein an undetectable hybridization signal indicates that Rupestris stem pitting associated virus is not present in the sample.
A method of determining whether a plant is infected with a Rupestris stem pitting associated virus, said method comprising the steps of:
- (a) providing a sample from said plant;
- and
- (b) contacting the sample with a nucleic acid probe comprising a Rupestris stem pitting virus nucleic acid molecule under conditions that allow for the hybridization of the probe to a Rupestris stem pitting associated virus nucleic acid molecule, wherein a detectable hybridization signal indicates that the plant is infected with Rupestris stem pitting associated virus.
A method of determining whether a plant is infected with a Rupestris stem pitting associated virus, said method comprising the steps of:
- (a) providing a sample from said plant;
- and
- (b) contacting the sample with a nucleic acid probe comprising a Rupestris stem pitting virus nucleic acid molecule under conditions that allow for the hybridization of the probe to a Rupestris stem pitting associated virus nucleic acid molecule, wherein an undetectable hybridization signal indicates that the plant is not infected with a Rupestris stem pitting associated virus.
Dependencies are inferred from the claim text.