Bee venom protein and gene encoding same
AssigneePan Pacific Pharmaceuticals, Inc.
InventorsXiangmin Cui, Yuefeng Lu
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Abstract
The invention provides a novel protein, PX3.101, which can be isolated from honey bee venom, antibodies against the polypeptide and nucleic acids encoding PX3.101 and fragments thereof. The invention also provides pharmaceutical compositions based upon PX3.101 polypeptide and methods for using same in the treatment of various diseases, including various inflammatory diseases such as rheumatoid arthritis.
Claims24
An isolated nucleic acid molecule that comprises a polynucleotide sequence that encodes a polypeptide having an amino acid sequence at least 75% identical to an amino acid sequence as set forth in SEQ ID NO:2 over a region at least about 40 amino acids in length when compared using the BLASTP algorithm with a wordlength (W) of 3, and the BLOSUM62 scoring matrix, wherein the polypeptide is effective to reduce the symptoms of an inflammatory disease.
An isolated nucleic acid molecule that comprises a polynucleotide sequence at least 75% identical to a nucleic acid sequence set forth in nucleotides 74 to 349 of SEQ ID NO:1 over a region of at least 50 nucleotides in length when compared using the BLASTN algorithm with a wordlength (W) of 11, M=5, and N=−4 and encodes a polypeptide that is effective in reducing the symptoms of an inflammatory disease.
A cell comprising a recombinant expression cassette comprising a promoter operably linked to a polynucleotide sequence which is at least about 75% identical to residues 74 to 349 of the polynucleotide sequence as set forth in SEQ ID NO:1 over a region at least about 50 nucleotides in length when compared using the BLASTN algorithm with a wordlength (W) of 11, M=5, and N=−4 and which encodes a polypeptide that is effective in reducing the symptoms of an inflammatory disease.
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of
- (a) a deoxyribonucleotide sequence complementary to nucleotides 74 to 349 of SEQ ID NO:1;
- (b) a ribonucleotide sequence complementary to nucleotides 74 to 349 of SEQ ID NO:1;
- (c) a nucleotide sequence complementary to the deoxyribonucleotide sequence of (a) or to the ribonucleotide sequence of (b);
- (d) a nucleotide sequence of at least 23 consecutive nucleotides that hybridizes to nucleotides 74 to 349 of SEQ ID NO:1;
- and
- (e) a nucleotide sequence that hybridizes to a nucleotide sequence of (d).
Dependencies are inferred from the claim text.