Method for the preparation of microspheres which contain colloidal systems
AssigneeOctoplus B.V.
InventorsWilhelmus Everhardus Hennink, Okke Franssen
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Abstract
The invention relates to a method for the preparation of microencapsulated colloidal systems such as liposomes, i.e., microspheres which comprise colloidal systems. These microencapsulated colloidal systems can be used as controlled release systems for the delivery of active ingredients in in vivo and in vitro applications. A method is provided in which the colloidal systems are added to a phase which comprises a water soluble crosslinkable polymer followed by formation of microspheres.
Claims29
A method for preparing microspheres encapsulating colloidal systems, which method comprises the steps of:
- a) providing an aqueous mixture of
- i) a first phase comprising a first water soluble crosslinkable polymer,
- ii) a second phase comprising a second water soluble polymer which is incompatible in solution with the polymer in said first phase, and
- iii) colloidal systems to be suspended in said first phase;
- b) forming an emulsion of said first phase in said second phase;
- c) forming microspheres in the emulsion by crosslinking at least part of said crosslinkable polymer, thus forming said microspheres encapsulating colloidal systems;
- and
- d) recovering the microspheres;
- wherein said colloidal systems are selected from the group consisting of liposomes, iscoms, polyplexes, lipoplexes, nanoparticles, solid lipid particles in the colloidal size range, emulsions, and combinations thereof.
Microspheres, at least 80 wt. % thereof having a particle diameter of between 100 nm and 100 μm, which microspheres are comprised of a degradable crosslinked polymer which encapsulates colloidal systems wherein said colloidal systems are selected from the group consisting of liposomes, iscoms, polyplexes, lipoplexes, nanoparticles, solid lipid particles in the colloidal size range, emulsions, and combinations thereof.
Dependencies are inferred from the claim text.