Sustained delivery of polyionic bioactive agents
AssigneeThe Children's Hospital of Philadelphia
InventorRobert J. Levy
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Abstract
The invention relates to compositions and methods for delivering a polyionic bioactive composition such as a nucleic acid to a tissue of an animal. The compositions of the invention include compositions which comprise a matrix comprising the polyionic bioactive agent and wherein at least most of the polyionic bioactive agent at the exterior portion of the matrix is present in a condensed form. The invention also includes methods of making such compositions, including particles, devices, bulk materials, and other objects which comprise, consist of, or are coated with such compositions. Methods of delivering a polyionic bioactive agent to an animal tissue are also described. The invention further includes a method of storing a nucleic acid.
Claims44
A composition for delivery of a nucleic acid, the composition comprising the nucleic acid and a matrix having an exterior portion, wherein at least most of the nucleic acid present at the exterior portion of the matrix is in a condensed form.
A method of making a composition for delivery of a nucleic acid, the method comprising
- providing a biodegradable matrix having an interior portion and an exterior portion, wherein the nucleic acid is suspended in the biodegradable matrix in a non-condensed form;
- and
- contacting the exterior portion of the biodegradable matrix with a polycationic condensing agent, whereby at least most of the nucleic acid assumes a condensed form at the exterior portion of the biodegradable matrix.
A method of making a composition for delivery of a nucleic acid, the method comprising
- providing a matrix which has an exterior portion and which comprises the nucleic acid at the exterior portion;
- and
- contacting the exterior portion of the matrix with a polycationic condensing agent, whereby at least most of the nucleic acid assumes a condensed form at the exterior portion of the matrix.
A method of delivering a nucleic acid to an animal tissue, the method comprising placing in fluid communication with the animal tissue a composition comprising the nucleic acid and a matrix having an exterior portion, wherein at least most of the nucleic acid is in a condensed form at the exterior portion of the matrix.
A kit comprising a biocompatible matrix having an exterior portion and an instructional material which describes combining the matrix with a nucleic acid and condensing at least most of the nucleic acid at the exterior portion of the matrix.
A kit comprising a composition comprising a nucleic acid and a biocompatible matrix having an exterior portion, wherein at least most of the nucleic acid present at the exterior portion of the matrix is in a condensed form, and an instructional material which describes administration of the composition to a tissue of an animal to effect delivery of the nucleic acid to the tissue.
A kit for coating an implantable device with a composition for delivery of a nucleic acid upon implantation of the device, the kit comprising a nucleic acid, a biocompatible polymeric matrix suspended in a solvent and a polycationic condensing agent, whereby at least most of the nucleic acid assumes a condensed form.
A kit for coating an implantable device with a composition for delivery of a nucleic acid upon implantation of the device, the kit comprising a nucleic acid, a suspension of monomers of a biocompatible polymeric matrix, a polymerization initiator, and a polycationic condensing agent, whereby at least most of the nucleic acid assumes a condensed form.
A method of storing a nucleic acid, the method comprising suspending the nucleic acid in a matrix and contacting the matrix with a polycationic condensing agent, whereby at least most of the nucleic acid in the matrix assumes a condensed form.
Dependencies are inferred from the claim text.