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AssigneesChiron Corporation, Washington University
InventorsThomas W. Dubensky, Jr., John M. Polo, Barbara A. Belli, Sondra Schlesinger, Sergey A. Dryga, Ilya Frolov
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Isolated nucleic acid molecules are disclosed, comprising an alphavirus nonstructural protein gene which, when operably incorporated into a recombinant alphavirus particle, eukaryotic layered vector initiation system, or RNA vector replicon, has a reduced level of vector-specific RNA synthesis, as compared to wild-type, and the same or greater level of proteins encoded by RNA transcribed from the viral junction region promoter, as is compared to a wild-type recombinant alphavirus particle. Also disclosed are RNA vector replicons, alphavirus vector constructs, and eukaryotic layered vector initiation systems which contain the above-identified nucleic acid molecules.
An alphavirus packaging cell line, comprising a host cell and a DNA alphavirus structural protein expression cassette, wherein said alphavirus structural protein expression cassette comprises a 5′ promoter that directs synthesis of an RNA transcript, a nucleic acid sequence encoding at least one alphavirus structural protein and a nucleic acid sequence encoding a selectable marker.
An alphavirus packaging cell comprising a cell and an alphavirus structural protein expression cassette, wherein said alphavirus structural protein expression cassette comprises:
Dependencies are inferred from the claim text.