Thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity
AssigneeAgency of Industrial Science and Technology
InventorsIkuo Matsui, Kazuhiko Ishikawa, Hiroyasu Ishida, Yoshitsugu Kosugi, Yasutaka Tahara
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Abstract
A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90° C. or higher, and the transformed host cell is cultured.
Claims29
An isolated thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity, wherein said thermostable enzyme is obtained from the hyperthermophilic archaeon Pyrococcus horikoshii, wherein said enzyme has an optimum temperature of about 90° C. to 100° C., and wherein said enzyme comprises amino acid residues 1 to 25 of SEQ ID NO:1.
An isolated thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity, wherein said enzyme is obtained from the hyperthermophilic archaeon Pyrococcus horikoshii, and wherein said enzyme has an optimum temperature of about 90° C. to 100° C., and has an optimum pH of about 8 to 9, wherein said enzyme is capable of acting on phosphatidylethanolamine, such that incorporation of a methyl group is detected only in phosphatidyl N-monomethylethanolamine and phosphatidyl N,N-dimethylethanolamine, and not phosphatidylcholine, and wherein said enzyme comprises amino acid residues 1 to 25 of SEQ ID NO:1.
An isolated thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity, wherein said enzyme is obtained from the hyperthermophilic archaeon Pyrococcus horikoshii, wherein said enzyme has an optimum temperature of about 90° C. to 100° C., and when said enzyme comprises the amino acid sequence of SEQ ID NO:1.
An isolated polypeptide sequence comprising SEQ ID NO:1.
A phosphatidylethanolamine N-methyltransferase enzyme isolated from Pyrococcus horikoshii, wherein said enzyme comprises amino acid residues 1 to 25 of SEQ ID NO:1.
An isolated thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity, wherein said thermostable enzyme is derived from the hyperthermophilic archaeon Pyrococcus horikoshii, and where said enzyme has an optimum temperature of about 90° C. to 100° C., an optimum pH of about 8 to 9, wherein said enzyme comprises amino acids 1 to 25 of SEQ ID NO:2, and said enzyme is capable of acting on phosphatidylethanolamine such that incorporation of a methyl group is detected in phosphatidyl N-monomethylethanolamine and phosphatidyl N,N-dimethylethanolamine, and not phosphatidylcholine, and wherein said enzyme is capable of acting on phosphatidyl N-monomethylethanolamine such that incorporation of a methyl group is detected in phosphatidyl N,N-dimethylethanolamine, and not phosphatidylcholine and not in phosphatidylcholine.
Dependencies are inferred from the claim text.