US 6,391,572 B1 · Methods for identifying modulators of transcriptional activator protein interactions

Abstract

The present invention relates to methods for identifying interacting regions of transcription factors, and methods for identifying agents which modulate the interactions, useful for affecting gene regulation, for example, cellular transformation. A site within residues 130-154 and within residues 343-358 in Stat3 were found to interact with the transcription factor c-Jun. On c-Jun, a site within residues 105 and 334, and more particularly, between 105 and 263, interact with Stat3. These sites of interactions permit methods for identifying agents which modulate the interaction between these transcription factors to modulate gene transcription.

Claims26

Dependent claims

A method for identifying an agent capable of modulating the interaction between c-Jun and a Stat protein comprising:

(a) providing a fragment of c-Jun extending from amino acid 105 to amino acid 334;
(b) providing a Stat3 protein fragment extending from amino acid 107 to amino acid 377;
(c) incubating a mixture of said c-Jun fragment and said Stat3 protein fragment with and without said agent;
(d) detecting the extent of binding between said c-Jun fragment and said Stat3 protein fragment in each of said mixtures;
and
(e) identifying an agent as capable of modulating said interaction as one which alters said extent of binding.

3 more elements

Dependencies are inferred from the claim text.

Methods for identifying modulators of transcriptional activator protein interactions

Life of this patent

Methods for identifying modulators of transcriptional activator protein interactions

Life of this patent

Abstract

Claims26