Gene encoding α-subunit of rice anthranilate synthase and DNA relating thereto
AssigneesHokko Chemical Industry Co., Ltd., National Agricultural Research Organization (NARO)
InventorsKyo Wakasa, Yuzuru Tozawa, Teruhiko Terakawa, Hisakazu Hasegawa
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Abstract
A DNA sequence is provided, which encodes an enzyme that is the α-subunit of the first isozyme of rice anthranilate synthase (ASA). Transformed plants having a high tryptophan content can be regenerated by culturing the plant cells having introduced therein such a recombinant vector which contains, at a position downstream of a promoter, an inserted DNA fragment carrying the above DNA sequence. In addition, methods of selecting transformed plant cells are disclosed, wherein plant cells transformed with the discloses DNA are resistant to inhibitory concentrations of tryptophan or tryptophan analogues.
Claims10
An isolated DNA encoding a protein having the amino acid sequence shown in SEQ ID NO:
- 13;
- said protein having the enzymatic activity of an α-subunit of a first isozyme of anthranilate synthase, said isozyme being insensitive to the feedback inhibition by tryptophan or a tryptophan analogue.
A recombinant vector which comprises a DNA sequence encoding a protein having the amino acid sequence shown in SEQ ID No. 13, wherein said DNA sequence is expressible in a host cell.
A recombinant vector which comprises an inserted DNA fragment comprising the nucleotide sequence shown in SEQ ID NO:
- 12, wherein said DNA sequence is expressible in a host cell.
An Escherichia coli as transformed with a recombinant vector which comprises an inserted DNA fragment comprising a DNA sequence encoding a protein having the amino acid sequence shown in SEQ ID No. 13, wherein said DNA sequence is expressible in said Escherichia coli.
An Escherichia coli transformed with a recombinant vector which comprises an inserted DNA fragment comprising the DNA sequence having the nucleotide sequence shown in SEQ ID NO:
- 12, wherein said DNA sequence is expressible in said Escherichia coli.
Dependencies are inferred from the claim text.