Bidirectional promoters and methods related thereto
AssigneeUniversity of Kentucky Research Foundation
InventorsSusheng Gan, Mingtang Xie, Yuehui He
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Abstract
The present invention comprises artificial nucleic acid constructs comprising a bidirectional promoter having minimal promoter and a common promoter, wherein said minimal promoters is operably linked to said common promoter, in opposite orientation to said common promoter, and 5′ to said common promoter. Those artificial nucleic acid constructs, wherein said bidirectional promoter further comprises at least one gene operably linked to said minimal promoter and said common promoter are preferred.
Claims12
An artificial nucleic acid construct, comprising a bidirectional promoter having a minimal promoter and a common promoter, each end of the bidirectional promoter operably linked to a polynucleotide coding for a polypeptide,
- (a) wherein said minimal promoter is operably linked to said common promoter, in opposite orientation to said common promoter, and 5′ to said common promoter;
- (b) wherein said bidirectional promoter further comprises a bidirectional promoter feature selected from the group consisting of said minimal promoter, and said common promoter;
- (b) wherein said minimal promoter is a minimal promoter of a promoter selected from the group consisting of 35S, PClSV OPR, and SAG12;
- and
- (c) wherein said common promoter is selected from the group consisting of 35S, PCISV, OPR, and SAG12.
A method of making a bidirectional promoter for use in plants, comprising
- (1) obtaining a polynucleotide comprising a plant promoter, wherein the plant promoter comprises a Common promoter region selected from the group consisting 35S, PCISV, OPR and SAG 12;
- (2) linking a minimal promoter, selected from the group consisting of 35S, PCISV, OPR and SAG12, and the common promoter to provide a recombinant polynucleotide, such that the recombinant polynucleotide comprises the common promoter operably linked with the minimal promoter, wherein
- (a) the minimal promoter is in opposite orientation to said common promoter, and;
- (b) the minimal promoter is 5′ to said common promoter.
Dependencies are inferred from the claim text.