Maize orthologues of bacterial RecA proteins
AssigneePioneer Hi-Bred International, Inc.
InventorsPramod B. Mahajan, Jinrui Shi, Christopher Baszczynski, John McElver, Benjamin Bowen
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Abstract
The present invention provides methods and compositions relating to altering RecA content and/or composition of plants. The invention provides isolated nucleic acids and their encoded proteins that are homologous to bacterial RecA genes. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The plant recA polynucleotides and their cognate products are useful for gene targeting in maize and other plant species and for use as a molecular marker.
Claims21
An isolated nucleic acid encoding a polypeptide with RecA activity comprising a member selected from the group consisting of:
- a) a first polynucleotide having at least 80% sequence identity to a second polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NOS:
- 2, 4, 6, 8, wherein the percent sequence identity is determined by the GAP algorithm under default parameters over the entire coding region of the second polynucleotide;
- and
- b) a polynucleotide which is fully complementary to said first polynucleotide of (a).
A recombinant expression cassette comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1, 3, 5, and 7, wherein said nucleic acid sequence is operably linked in antisense orientation to a promoter.
An isolated nucleic acid comprising a polynucleotide of at least 50 nucleotides in length which selectively hybridizes under stringent conditions and a wash of 0.1×SSC, 0.1% SDS at 65° C. to a nucleic acid selected from the group consisting of SEQ ID NOS:
- 1, 3, 5, and 7 or a nucleic acid fully complementary thereof.
An isolated nucleic acid comprising a polynucleotide encoding a polypeptide with RecA activity, said polynucleotide having at least 80% sequence identity to a polynucleotide selected from the group consisting of SEQ ID NOS, 1, 3, 5, and 7 or a nucleic acid fully complementary thereof, wherein the percent sequence identity is determined by the GAP algorithm under default parameters over the entire coding region.
Dependencies are inferred from the claim text.