MAR/SAR elements flanking RSYN7-driven construct
AssigneePioneer Hi-Bred International, Inc.
InventorsWesley B. Bruce, Sheila E. Maddock
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Abstract
This invention discloses to compositions and methods for altering the characteristic pattern of expression associated with a promoter. More particularly the constitutive expression pattern associated with the small synthetic promoter, Rsyn7 is modified so that expression of a heterologous nucleotide sequence operably linked to the Rsyn7 promoter is in a tissue localized manner. This modification of the Rsyn7 pattern of expression occurs as a result of the addition of matrix attachment region DNA sequences to the flanks or 5′ and 3′ ends of an expression cassette comprising the Rsyn7 promoter operably linked to a heterologous nucleotide sequence of interest. DNA constructs, transformed plant cells and transformed plants are provided.
Claims26
A DNA construct comprising a promoter-driven construct wherein said promoter-driven construct comprises:
- (a) an Rsyn7 promoter comprising the nucleotide sequence set forth in SEQ ID NO:
- 1 or 2;
- (b) a nucleotide sequence operably linked to said promoter;
- (c) a transcription and translation termination region;
- and
- (d) maize ADH1 matrix attachment region DNA sequences flanking the combined elements (a), (b), and (c).
A method for altering the expression pattern of an Rsyn7 promoter construct, said method comprising operably linking at least one maize ADH1 matrix attachment region to said construct, said construct comprising an Rsyn7 promoter comprising the sequence set forth in SEQ ID No:
- 1 or 2, a heterologous DNA sequence operably linked to said promoter, and a transcription and translation termination region, wherein said matrix attachment region alters the expression pattern of said promoter.
A DNA construct comprising the following operably linked elements:
- (a) an Rsyn7 promoter comprising the nucleotide sequence set forth in SEQ ID NO:
- 1 or 2;
- (b) a nucleotide sequence of interest;
- (c) a transcription and translation termination region;
- and, (d) at least one maize ADH1 matrix attachment region DNA sequence.
A plant having stably incorporated into its genome a DNA construct comprising the following operably linked elements:
- (a) an Rsyn7 promoter comprising the nucleotide sequence set forth in SEQ ID NO:
- 1 or 2;
- (b) a nucleotide sequence of interest;
- (c) a transcription and translation termination region;
- and
- (d) at least one maize ADH1 matrix attachment region DNA sequence.
Dependencies are inferred from the claim text.