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US 6,388,058 B1Granted
Method of purifying daunomycin
AssigneeMercian Corporation
InventorsOsamu Johdo, Konomi Iguchi, Takeo Yoshioka
Life of this patent
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Abstract
The present invention relates to a method in which daunomycin is eluted from a hydrophobic porous synthetic resin carrier onto which daunomycin is adsorbed from crude daunomycin with a buffered aqueous solution containing a water-miscible organic solvent to recover purified daunomycin. Daunomycin (daunorubicin) is purified efficiently.
Claims10
A purifying method of daunomycin comprising:
- eluting daunomycin from a hydrophobic porous synthetic resin carrier, wherein daunomycin is adsorbed onto said resin carrier from crude daunomycin, with an eluting buffered aqueous solution containing a water-miscible organic solvent which is buffered to a pH of about 3.25 to 6, and
- recovering purified daunomycin having a relative purity of at least 98% from the eluate;
- wherein said organic solvent is at least one solvent selected from the group consisting of methanol and ethanol.
A purifying method of daunomycin comprising:
- eluting daunomycin from a hydrophobic porous synthetic resin carrier, wherein daunomycin is adsorbed onto said resin carrier from crude daunomycin, with an aqueous solution containing a water-miscible organic solvent which is buffered to a pH of about 2.5 to about 5.5, and
- recovering purified daunomycin having a relative purity is 80% or more from the eluate;
- wherein said organic solvent is at least one solvent selected from the group consisting of methanol and ethanol.
Dependencies are inferred from the claim text.