Polymeric adsorbents and method of preparation
AssigneeRohm and Haas Company
InventorsKarl Chaplin Deissler, Marlin Kenneth Kinzey, John Joseph Maikner, Robert E. Rosen
Life of this patent
Estimating the term…
Abstract
Macroporous polymers having selected porosity and permeability characteristics that provide rigid polymer matrices suitable for use in medium and high pressure reversed phase liquid chromatography (RPC) are disclosed. A method for preparing the polymers using selected mixed porogens in selected proportions relative to the monomer phase is also disclosed. The polymers are especially useful as stationary phases in large scale chromatography columns without developing increased pressures during prolonged use, while maintaining good chromatographic performance for targeted biomolecules, such as insulin.
Claims19
A macroporous polymer comprising polymerized monomer units of:
- (a) 50 to 100 percent by weight of one or more polyvinylaromatic monomer, and
- (b) zero to 50 percent by weight of one or more monounsaturated vinylaromatic monomer;
- wherein the polymer has:
- (i) a total porosity of 0.7 to 2 cubic centimeter per gram;
- (ii) an operational mesoporosity of 0.7 to 1.9 cubic centimeter per gram;
- (iii) an average particle size diameter of 2 to 600 microns;
- (iv) a surface area of 200 to 1500 square meters per gram;
- (v) a flow resistance value from 700 to less than 1,800 at 10 bar pressure and from 1,500 to less than 7,000 at 60 bar pressure;
- and
- (vi) a total insulin capacity of 75 to 150 grams insulin/liter of polymer and a dynamic insulin capacity of 60 to 150 grams insulin/liter of polymer.
Dependencies are inferred from the claim text.