Purification method
AssigneeNational Blood Authority
InventorsJohn Edward More, Jacqueline Rott, David Roger Lewin
Life of this patent
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Abstract
The invention relates to a method of removing endotoxin from preparations of alpha-1-acid glycoprotein (orosomucoid) by contact with a finely divided non-toxic resin such as fumed silica. The invention also relates to a purification process for alpha-1-acid glycoprotein which includes this deprogenation step, and to the depyrogenated product and its clinical uses.
Claims17
A method of removing lipopolysaccharide (LPS) from an alpha-1-acid glycoprotein (AAG) containing preparation comprising contacting said preparation with a finely divided inorganic non-toxic resin, to produce an AAG preparation having a LPS concentration of less than or equal to 0.1 Eu/mg AAG.
A method of purifying AAG comprising contacting an AAG-containing preparation with an anion exchange matrix, eluting an AAG-enriched fraction from said matrix and depyrogenating an AAG-enriched fraction by contact with a finely divided inorganic non-toxic particulate resin followed by separation of an LPS-depleted AAG fraction, to produce an AAG preparation having a LPS concentration of less than or equal to 0.1Eu/mg AAG.
Dependencies are inferred from the claim text.