MAGE-10 encoding CDNA, the tumor rejection antigen precursor MAGE-10, antibodies specific to the molecule, and uses thereof
AssigneeLudwig Institute for Cancer Research
InventorsDonata Rimoldi, Victor Jongeneel, Pierre Coulie, Jean-Charles Cerrottini, Stefan Carrel, Daryl Reed
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Abstract
Isolated cDNA molecules which encode the tumor rejection antigen precursor MAGE-10, the protein itself, antibodies to it, and uses of these are part of the invention.
Claims3
Polyclonal antiserum obtained by immunizing a non-human animal with the isolated MAGE-10 tumor rejection antigen precursor having a molecular weight of about 72 kD as determined by SDS PAGE, encoded by an isolated nucleic acid molecule, the complementary sequence of which hybridizes to nucleotides 164-545 of SEQ ID NO:3 in 0.2×SSC, 0.1% SDS at 65° C. under conditions favoring an immune response thereto, and isolating antiserum produced thereby.
Hybridoma cell which produces monoclonal antibody which binds to an isolated MAGE -10 tumor rejection antigen precursor having a molecular weight of about 72 kD as determined by SDS PAGE, encoded by an isolated nucleic acid molecule, the complementary sequence of which hybridizes to nucleotides 164-545 of SEQ ID NO:3 in 0.2×SSC, 0.1% SDS at 65° C.
A monoclonal antibody, which binds to an isolated MAGE-10 tumor rejection antigen precursor having a molecular weight of about 72 kD as determined by SDS PAGE, encoded by an isolated nucleic acid molecule, the complementary sequence of which hybridizes to nucleotides 164-545 of SEQ ID NO:3 in 0.2X SSC, 0.1% SDS at 65° C., wherein said antibody is humanized.
Dependencies are inferred from the claim text.