Photo-induced electron transfer fluorescent sensor molecules
AssigneeBeckman Coulter, Inc.
InventorsSusumu Arimori, Tony D. James
Life of this patent
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Abstract
Disclosed is a modular fluorescence sensor having the following general formula: where Fl is a fluorophore, N is a nitrogen atom, Bd1 and Bd2 are independently selected binding groups, Sp is an aliphatic spacer, and An is an anchor group for attaching the sensor to solid substrates. n=1 or 2, m=1 or 2, x is an integer, and y=1 or 2. The binding groups are capable of binding an analyte molecule to form a stable 1:1 complex. In a preferred embodiment, the Bd1 is R1—B(OH)2 and Bd2 is R2—B(OH)2. R1 and R2 are aliphatic or aromatic functional groups selected independently from each other and B is a boron atom. The present invention also provides methods of synthesizing a modular fluorescence sensor and its use in labeling solid substrates.
Claims28
A modular fluorescence sensor having the following general formula:
- wherein:
- Fl is a fluorophore;
- N is a nitrogen atom;
- Bd1 and Bd2 are independently selected binding groups, wherein the binding groups are capable of binding an analyte molecule to form a stable 1:1 complex;
- Sp is an aliphatic spacer;
- An is an anchor group for attaching the sensor to a solid substrate;
- and
- n, m, x and y are integers, where n=1 or 2, m=1 or 2, and y=1 or 2, x is an integer.
A modular fluorescence sensor having the following general formula:
- wherein:
- Fl is a fluorophore;
- N is a nitrogen atom and B is a boron atom;
- R1 and R2 are aliphatic or aromatic functional groups which allow covalent binding of an analyte to the hydroxyl groups forming a stable 1:1 complex, wherein R1 and R2 are selected independently from each other and;
- An is an anchor group for attaching the sensor to a solid substrate;
- and
- x is an integer.
A modular fluorescence sensor of the following formula:
A composition comprising:
- a modular fluorescence sensor having the following general formula:
- wherein:
- Fl is a fluorophore;
- N is a nitrogen atom;
- Bd1 and Bd2 are independently selected binding groups, wherein the binding groups are capable of binding an analyte molecule to form a stable 1:1 complex;
- Sp is an aliphatic spacer;
- An is an anchor group for attaching the sensor to a solid substrate;
- and
- n, m, x and y are integers, were n=1 or 2, m=1 or 2, and y=1 or 2, x is an integer;
- and
- an analyte bound to the sensor.
A composition comprising:
- a modular fluorescence sensor having the following general formula:
- wherein:
- Fl is a fluorophore;
- N is a nitrogen atom;
- B is a boron atom;
- R1 and R2 are aliphatic or aromatic functional groups which allow covalent binding of an analyte to the hydroxyl groups forming a stable 1:1 complex, wherein R1 and R2 are selected independently from each other;
- An is an anchor group for attaching the sensor to a solid substrate;
- and
- x is an integer;
- and
- glucose bound to the sensor.
Dependencies are inferred from the claim text.