Method for producing recombinant virus
AssigneeAventis Pharma S.A.
InventorsHélène Leblois-Prehaud, Michel Perricaudet, Emmanuelle Vigne, Patrice Yeh
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Abstract
The invention concerns a method for producing recombinant virus. This method is based on the use of baculovirus for providing the complementary functions. It also concerns constructs used for implementing this method, the producing cells, and the resulting virus.
Claims27
A process for producing a defective recombinant adenovirus, the process comprising introducing into a competent cell a genome of a defective recombinant adenovirus and a baculovirus comprising a function necessary for transcomplementation of the defective adenoviral genome, wherein the defective recombinant adenoviral genome is introduced into the competent cell by transfection.
The process according to claim 3, wherein the defective recombinant adenoviral genome is introduced into the competent cell using a recombinant baculovirus.
A process for producing a defective recombinant virus, the process comprising introducing into a competent cell a genome of a defective recombinant virus and a baculovirus comprising one or more of the functions necessary for transcomplementation of the genome, wherein the virus is a defective recombinant adenovirus.
A recombinant baculovirus comprising, inserted into its genome, a DNA region flanked by two nucleic acid sequences which cause site-specific recombination and which are positioned in direct orientation, wherein the DNA region comprises a replication origin functional in a competent cell and a nucleic acid encoding a complementation function of a virus, and wherein the two nucleic acid sequences which cause site-specific recombination are LoxP sequences of P1 bacteriophage.
A recombinant baculovirus comprising within its genome a nucleic acid encoding a complementation function of a defective virus under the control of a heterologous promoter, wherein the nucleic acid encoding the complementation function corresponds to a fragment of a canine adenovirus genome.
A recombinant baculovirus comprising within its genome a nucleic acid encoding a complementation function of a defective virus under the control of a heterologous promoter, wherein the complementation function is selected from the group consisting of functions encoded by adenovirus E1, E2, E4, late genes L1 to L5, pIX, and Iva2 regions, wherein the promoter comprises a promoter region naturally responsible for the expression of the complementation function.
A process for producing a defective recombinant adeno-associated virus, the process comprising introducing into a competent cell a genome of a defective recombinant adeno-associated virus and a baculovirus comprising a function necessary for transcomplementation of the defective adenoviral genome, wherein the defective recombinant adeno-associated viral genome is introduced into the competent cell by transfection.
Dependencies are inferred from the claim text.