Process for producing cytidine diphosphate choline
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsAkihiko Maruyama, Tatsuro Fujio, Sadao Teshiba
Life of this patent
Estimating the term…
Abstract
A process for producing cytidine diphosphate choline is provided. The process comprises carrying out an enzymatic reaction using microorganisms having the enzyme activities of cytidine-5′-triphosphate synthetase (pyrG), cholinephosphate cytidylyltransferase (CCT) and choline kinase (CKI) and a microorganism capable of producing uridine-5′-triphosphate from orotic acid as the enzyme sources, and orotic acid and choline and/or phosphorylcholine as the substrates; allowing cytidine diphosphate choline to accumulate in the reaction mixture; and recovering cytidine diphosphate choline from said reaction mixture.
Claims9
A process for producing cytidine diphosphate choline, which comprises contacting an enzymatically active treatment product of a culture of a microorganism which carries a recombinant DNA comprising a DNA fragment encoding pyrG, CCT and CKI and a vector (hereinafter referred to as Microorganism A1) and an enzymatically active treatment product of a culture of another microorganism which has orotate phosphoribosyltransferase, OMP decarboxylase, nucleosidemonophosphate kinase and nucleosidesiphosphate kinase activities (hereinafter referred to as Microorganism B) with orotic acid and choline in a liquid medium;
- allowing cytidine diphosphate choline to accumulate in the liquid medium;
- and recovering cytidine diphosphate choline from the liquid medium.
A process for producing cytidine diphosphate choline, which comprises contacting an enzymatically active treatment product of a culture of a microorganism which carries a recombinant DNA comprising a DNA fragment encoding pyrG and CCT and a vector (hereinafter referred to as Microorganism A2) and an enzymatically active treatment product of a culture of another microorganism which as orotate phosphoribosyltransferase, OMP decarboxylase, nucleosidemonophosphate kinase and nucleosidediphosphate kinase activities (hereinafter referred to as Microorganism B) with orotic acid and phosphorylcholine in a liquid medium;
- allowing cytidine diphosphate choline to accumulate in the liquid medium;
- and recovering cytidine diphosphate choline from the liquid medium.
Dependencies are inferred from the claim text.