Process for producing S-hydroxynitrile lyase
AssigneeNippon Shokubai Co., Ltd.
InventorHisashi Semba
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Abstract
The present invention provides a process for producing S-hydroxynitrile lyase, comprising the steps of culturing in a medium yeast cells transformed with recombinant DNA comprising an expression vector into which a S-hydroxynitrile lyase (EC 4.1.2.37) coding gene derived from cassava (Manihot esculenta) has been incorporated, and collecting S-hydroxynitrile lyase from the yeast cells. According to the present invention, a large amount of S-hydroxynitrile lyase can be efficiently produced by genetic engineering techniques.
Claims13
A process for producing S-hydroxynitrile lyase, comprising the steps of:
- culturing in a medium yeast cells transformed with recombinant DNA comprising an expression vector into which S-hydroxynitrile lyase (EC 4.1.2.37) coding gene from cassava (Manihot esculenta) has been incorporated, and collecting S-hydroxynitrile lyase from the yeast cells.
A process for producing S-hydroxynitrile lyase, comprising the steps of culturing in a medium a yeast cell transformed with recombinant DNA comprising a yeast episome expression vector into which S-hydroxynitrile lyase (EC4.1.2.37) coding gene from cassava (Manihot esculenta) is incorporated, and collecting S-hydroxynitrile lyase from the yeast cells.
A process for producing S-hydroxynitrile lyase in yeast, comprising the steps of:
- culturing in a medium the yeast Saccharomyces transformed with recombinant DNA comprising a yeast integrating expression vector into which S-hydroxynitrile lyase (EC 4.1.2,37) coding gene from cassava (Manihot esculenta) is incorporated, and collecting S-hydroxynitrile lyase from the yeast cells.
A process for producing S-hydroxynitrile lyase in yeast, comprising the steps of:
- culturing in a medium the yeast Pichia transformed with recombinant DNA comprising a yeast integrating expression vector into which S-hydroxynitrile lyase (EC 4.1.2.37) coding gene from cassava (Manihot esculenta) is incorporated, and collecting S-hydroxynitrile lyase from the yeast cells.
Dependencies are inferred from the claim text.