Bacterial strains and fermentation processes for the production of 2-keto-l-gulonic acid
AssigneeArcher Daniels Midland Company
InventorsHungming J. Liaw, Robert L. Kowzic, Jr., John M. Eddington, Yuequin Yang
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Abstract
The present invention relates to novel bacterial strains belonging to the genera Gluconobacter, Ketogulonogenium and Bacillus useful for the production of 2-keto-L-gulonic acid. The present invention further relates to the use of these strains for the production of 2-keto-L-gulonic acid in mixed culture by fermentative conversion of D-sorbitol. The present invention further relates to the strains of the present invention transformed by a vector.
Claims36
A biologically pure culture of a microorganism strain comprising all of the identifying characteristics of a strain selected from the group consisting of NRRL B-30265, NRRL B-30266, NRRL B-30267 and NRRL B-30268, or a mutant thereof derived from said strain.
A microorganism culture system comprising a mixture formed from a biologically pure culture of a microorganism strain having all of the identifying characteristics of strain NRRL B-30266 and a biologically pure culture of a microorganism strain having all of the identifying characteristics of strain NRRL B-30265, wherein said culture system is capable of producing at least about 40 g/L of 2-keto-L-gulonic acid from D-sorbitol.
A process for the production of 2-keto-L-gulonic acid which comprises:
- (a) culturing a microorganism strain comprising all of the identifying characteristics of NRRL B-30265 or a mutant thereof in mixed culture with a microorganism strain capable of converting D-sorbitol to L-sorbose in a medium containing D-sorbitol, for a time sufficient for said D-sorbitol to be converted to 2-keto-L-gulonic acid;
- and
- (b) recovering said 2-keto-L-gulonic acid,
- wherein said mutant is capable of producing at least about 40 g/L of 2-keto-L-gulonic acid in mixed culture.
Dependencies are inferred from the claim text.