Method of identifying and quantifying specific fungi and bacteria
AssigneeU.S. Environmental Protection Agency
InventorsRichard Haugland, Stephen Vesper
Life of this patent
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Abstract
Fungi and bacteria can be detected and rapidly quantified by using the nucleotide sequences taught here that are specific to the particular species or group of species of fungi or bacteria. Use of the sequences can be made with fluorescent labeled probes, such as in the TaqMan™ system which produces real time detection of polymerase chain reaction (PCR) products. Other methods of detection and quantification based on these sequences include hybridization, conventional PCR or other molecular techniques.
Claims79
A method for detecting and quantifying specific fungi or bacteria in a sample comprising:
- a. extracting and recovering DNA from the organism in the sample;
- b. hybridizing and amplifying the DNA sequences such that the specific fungus or bacterium can be identified and quantified without interference from DNA in the sample belonging to other fungi or bacteria.
The method according to claim wherein the bacteria are selected from the group consisting of Helicobacter pylorii, the forward primer is HpylF1:
- 5′-GGGTATTGAAGCGATGTTTCCT (SEQ ID NO:223), the reverse primer is HpylR1:
- 5′-GCTTTTTTGCCTTCGTTGATAGT (SEQ ID NO:224), and the probe is HpylP1:
- 5′-AAACTCGTAACCGTGCATACCCCTATTGAG (SEQ ID NO:225).
Dependencies are inferred from the claim text.