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US 6,387,649 B1Granted
Assay for a regulator of cell cycle progression
AssigneeProlifix Limited
InventorsNicholas B. La Thangue, Susana De La Luna
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Abstract
The present invention provides nuclear localisation signals derived from the DP-3 and E2F-1 transcription factors and the use of these signals in assays for regulators of cell cycle progression. Such assays involve using the signals to direct a marker gene to the nucleus and determining whether the nuclear localisation of the marker is disrupted by the presence of a putative regulator.
Claims1
An assay for a regulator of cell cycle progression which comprises:
- (a) expressing in a cell a protein which is an E2F-1 transcription factor or portion thereof, which protein comprises a functional nuclear localization signal and which protein is capable of forming a heterodimer with a DP transcription factor or portion thereof;
- (b) expressing in said cell the DP transcription factor or portion thereof, wherein the DP transcription factor is selected from the group consisting of DP-3β and DP-3γ;
- and
- (c) determining the degree of nuclear localization of the protein in the presence and absence of a putative regulator, wherein a difference in the degree of nuclear localization in the presence and absence of the putative regulator indicates that the putative regulator is a regulator of cell cycle progression.
1 more element
Dependencies are inferred from the claim text.