Methods and materials relating to novel CD39-like polypeptides
AssigneeHyseq, Inc.
InventorsJohn Ford, Julio J. Mulero
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Abstract
The invention provides novel polynucleotides isolated from cDNA libraries of human fetal liver-spleen and macrophage as well as polypeptides encoded by these polynucleotides and mutants or variants thereof. The polypeptides correspond to a novel human CD39-like protein. Other aspects of the invention include vectors containing polynucleotides of the invention and related host cells as well a processes for producing novel CD39-like polypeptides, and antibodies specific for such polypeptides.
Claims18
A method of identifying an inhibitor of a nucleotide diphosphatase (NDPase) polypeptide comprising an amino acid sequence with at least about 90% sequence identity to SEQ ID NO:
- 3 or the mature protein portion thereof comprising the steps of:
- (a) contacting said NDPase polypeptide with a nucleotide diphosphate (NDP) substrate in the presence and absence of a test compound:
- (b) measuring NDPase activity of said NDPase polypeptide in the presence and absence of the test compound;
- and
- (c) identifying the test compound as an inhibitor when NDPase activity of said NDPase polypeptide is decreased in the presence of the test compound.
Dependencies are inferred from the claim text.