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US 6,387,644 B1Granted
Motor proteins and methods for their use
AssigneeCytokinetics, Inc.
InventorChristophe Beraud
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Abstract
The present invention provides high throughput screening systems for identifying compounds useful in the treatment of cellular proliferation disorders. The method can be performed in plurality simultaneously with fluorescence or absorbance readouts.
Claims5
A method of identifying a candidate agent as a modulator of function of a target protein wherein said target protein comprises SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8 and said method comprises:
- a) adding a candidate agent to a mixture comprising the target protein that directly or indirectly produces ADP or phosphate under conditions which normally allow the production of ADP or phosphate;
- b) subjecting the mixture to a reaction that uses said ADP or phosphate as a substrate under conditions which normally allow the ADP or phosphate to be utilized;
- and
- c) determining the level of activity of the reaction wherein a change in said level between the presence and absence of said candidate agent indicates a modulator of said target protein function.
1 more element
Dependencies are inferred from the claim text.