Human platelet heparanase polypeptides, polynucleotide molecules that encode them, and methods for the identification of compounds that alter heparanase activity
AssigneePharmacia & Upjohn Company
InventorsRobert Leroy Heinrikson, Michael B. Fairbanks, Ana M. Mildner
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Abstract
The present invention provides isolated human heparanase polypeptides, and the isolated polynucleotide molecules that encode them, as well as vectors and host cells comprising such polynucleotide molecules. The invention also provides a method for the identification of an agent that alters heparanase activity.
Claims10
An isolated human heparanase polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence of a human pre-proheparanase polypeptide having the complete amino acid sequence of SEQ ID NO:2;
- (b) an amino acid sequence of a human proheparanase polypeptide having the amino acid sequence at residues 23 through 530 of SEQ ID NO:2;
- (c) an amino acid sequence of the 8 kDa subunit of human heparanase having amino acid sequence at residues 23 through 96 of SEQ ID NO:2;
- and
- (d) an amino acid sequence of the 56 kDa subunit of human heparanase having the amino acid sequence at residues 145 through 530 of SEQ ID NO:2.
A human heparanase enzyme comprising
- (a) an isolated human heparanase polypeptide comprising the amino acid sequence at residues 145 through 530 of SEQ ID NO:2;
- and
- (b) an isolated human heparanase polypeptide comprising the amino acid sequence at residues 23 through 96 of SEQ ID NO:2.
A human heparanase enzyme comprising
- (a) an isolated human heparanase polypeptide that is encoded by an isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a polynucleotide having the nucleotide sequence at residues 433 through 1590 of SEQ ID NO:1;
- and
- (b) an isolated human heparanase polypeptide that is encoded by an isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a polynucleotide having the nucleotide sequence at residues 67 through 288 of SEQ ID NO:1.
An isolated human heparanase wherein said polypeptide comprises an amino acid sequence of the 56 kDa subunit of human heparanase polypeptide having the amino acid sequence at residues 145 through 530 of SEQ ID NO:2.
Dependencies are inferred from the claim text.